In Vitro Fertilization (IVF)
In vitro fertilization (IVF) is the Mixing of a woman’s egg and a man’s sperm in
a laboratory dish. In vitro means outside the body. Fertilization means the sperm
has attached to and entered the egg.
IVF is a form of assisted reproductive technology (ART). This means special medical
techniques are used to help a woman become pregnant. It is most often tried when
other, less expensive fertility techniques have failed.
There Are Five Basic Steps To IVF:
Step 1: Stimulation, also called super ovulation
Hormones (Gonadotropins), are given to the woman to boost egg production.
Normally, a woman produces one egg per month. Fertility drugs tell the ovaries to
produce several eggs.
During this step, the woman will have regular transvaginal ultrasounds to examine
the ovaries and blood tests to check hormone levels.
Step 2: Egg retrieval
A minor surgery, called follicular aspiration, is done to remove the eggs from the
The surgery is done as an outpatient procedure at our clinic most of the time. The
woman will be given Mild anaesthesia so she does not feel pain during the procedure.
Using ultrasound images as a guide, the health care provider inserts a thin needle
through the vagina and into the ovary and sacs (follicles) containing the eggs.
The needle is connected to a suction device, which pulls the eggs and fluid out
of each follicle, one at a time.
The procedure is repeated for the other ovary. There may be some cramping after
the procedure, but it will go away within a day.
In rare cases, a pelvic laparoscopy may be needed to remove the eggs.
The problem with IVF can be less percentage of fertilisation. With ICSI we are making
sure that we inject one sperm in each egg and so fertilisation rate is supposed
to be better with ICSI.
So there shouldn’t be a standard protocol for IVF or ICSI to be done, it should
be decided by embryologist in the best interest of the patient.
Whenever patients are having very bad quality sperms or very low motility then embryologist’s
work is very crucial in creating the good quality embryos. even if one million sperms
are there that means few sperms from that 10 lac sperms to be choosen for ICSI,
so embryologiat’s training, education, qualification and experience makes a whole
lot of difference in the outcome of positive or negative results.
In IVF , we mix the sperms and eggs in one dish and culture them. In ICSI, we choose
one sperm, immobile and catch the sperm and we inject the sperm inside the egg.
Here we don’t wait for the sperm to enter the egg on its own but we do inject each
egg with one sperm.